Lactic acid bacteria (LAB) is a functionally related group of bacteria comprising the families Aerococcaceae, Carnobacteriaceae, Enterococcaceae, Lactobacillaceae, Leuconostocaceae and Streptococcaceae, which are phylogenetically diverse. They have a highly fermentative lifestyle, converting a range of sugars into mainly lactic acid. LAB play an important role in different forms of food-related biotechnology. They are gaining attention towards novel uses due to their safety for human and animal consumption, metabolic versatility and wide ecological niche adaptation (including industrial-scale fermentations).
Candida albicans is the most prevalent opportunisti human fungal pathogen. The systemic candidiasis is associated with high morbidity and mortality rates. Understanding differences between C. albicans and mammalian molecular biology is critical to development of the next generation of antifungal therapeutics. This requires investigators to be able to quickly and precisely manipulate the genome of C. albicans.
Creative Biogene is a leading biotechnology company offering the best Pichia pastoris genome editing services. With years of experience and expertise in microbial genome editing, our talented scientists will work closely with you to provide any help in Pichia pastoris genome editing services.
Pichia pastoris (Komagataella phaffii), Due to its potent secretion of pure protein, less extensive glycosylation and high cell density growth, is one of the most commonly used expression systems for heterologous protein production. It has been widely used for protein production in basic and applied research.
This product is a pseudovirus (PsV) of HPV type 6 (HPV6) that is produced from 293TT cells by co-transfection of plasmids expressing L1 and L2 proteins from HPV6 as well as a GFP marker. HPV6 PsV can infect into cells and express GFP but can not replicate in the transduced cells because it does not contain HPV6 viral genome DNA. HPV6 PsV is a useful tool that can be used for in vitro HPV neutralization assay. HPV6
Gene knockout cell lysates are the cell homogenate in RIPA buffer made with double-knockout cell lines. The Gene knockout cell lines are developed by CRISPR. The protein concentration was determined with BCA assay. A vial of lysate from the parental cell line was also included as an internal control. KRT7 KO Cell Lysate
Gene knockout cell lysates are the cell homogenate in RIPA buffer made with double-knockout cell lines. The Gene knockout cell lines are developed by CRISPR. The protein concentration was determined with BCA assay. A vial of lysate from the parental cell line was also included as an internal control. VIM KO Cell Lysate-HeLa
Creative Biogene is one of the leading biotechnology companies which has the expertise and ability in providing oligonucleotide synthesis service for customers worldwide. Creative Biogene's sophisticated equipments, advanced technologies and highly experienced staffs are available to provide you a full spectrum of high quality specialty oligonucleotide synthesis service ranging from oligo synthesis for research and GMP grade, modified oligonucleotides to high throughput DNA or RNA oligo synthesis.
Creative Biogene is one of the leading biotechnology companies which has the expertise in providing gene mutagenesis service for customers. Creative Biogene's sophisticated equipments, advanced technologies and highly experienced staffs are available to provide you with the complete mutagenesis service, including creating single or multiple point mutations, deletions, insertions or random mutations into any target gene. Using fast and efficient mutagenesis methods, Creative Biogene can quickly create novel constructs for large or small projects.
Lentiviruses are very efficient at delivering genetic materials to both dividing and non-dividing cells. According to whether proviral DNA is formed in transduced cells, lentiviruses can be further divided into two groups: integrating lentivirus (ILV) and integrase-deficient lentivirus (IDLV). ILV is first developed and applied in scientific research and IDLV is constructed later through genetic modification.
The application of CRISPR libraries makes large-scale genome editing and screening in basic research a reality. Creative Biogene helps clients unlock the full potential of their genetic research with our advanced CRISPR screening services. We offer a large number of CRISPR libraries to meet the research demands of different customers, including human whole-genome knockout libraries, mouse whole-genome knockout libraries, pathway-associated gRNA libraries, CRISPRa libraries, CRISPRi libraries, and customized gRNA libraries. Our cutting-edge technology enables high-throughput and scalable screening of genome-wide libraries for rapid identification of novel gene functions, discovery of gene interactions and exploration of complex signaling pathways.
Transfection alters the genetic makeup of eukaryotic cells by introducing foreign nucleic acids, including DNA, RNA, and small noncoding RNAs such as siRNA, shRNA, and miRNA. Stable transfection results in sustained, long-term expression, whereas expression is eventually lost following transient transfection as the host cell replicates. Scientists apply stable transfection to long-term and large-scale genetic and pharmacological studies. Transient transfection can be used for short-term studies, such as studying the effects of gene knock-in or knock-down.
Reporter stable cell lines serve as invaluable tools for scientific research, facilitating the expression of reporter proteins engineered through the integration of reporter expression cassettes into cell genomes using plasmid or lentivirus vectors. Commonly utilized in life science and biotechnology studies, these reporter genes, such as green fluorescent protein (GFP), red fluorescent protein (RFP), luciferase, and secreted alkaline phosphatase (SEAP), offer visual identification.
Gene expression knockdown through RNA interference (RNAi) is a widely used method for studying gene function. Small interfering RNA (siRNA) is commonly employed for transient knockdown in mammalian cell culture due to its quick and efficient transfection capabilities. However, siRNA has limitations in low transfection efficiency cell types and experiments requiring prolonged knockdown. An alternative method is short hairpin RNA (shRNA), a synthetic non-coding RNA utilizing endogenous microRNA machinery for functional RNAi.shRNA, forming a tight hairpin turn, silences gene expression via RNAi. Delivery can be achieved through plasmids, viral vectors, or bacterial vectors. Compared to siRNA, shRNA offers advantages in longevity, delivery options, and cost.
Creative Biogene is dedicated to optimize the plant suspension cell-based protein expression platform for years, to product recombinant proteins, polypeptides and industrial proteins for global customers. With years of experience in plant tissue culture and protein expression, Creative Biogene provides a comprehensive service ranging from codon optimization, molecular construction, plant cell transformation to protein expression and purification. Our mission is to help you manufacture complex proteins, antibodies, and vaccines using unique plant expression platform.
Creative Biogene is one of the pioneers offering Pseudovirus-related services with tested and proven protocols. As the industry leader, Creative Biogene has rich history of success and commitment to providing our clients with the best service using only state-of-the-art equipment and finest materials. Coming from world renowned institutions around the globe, researchers of our laboratory team are well-trained and highly experienced with the devotion and dedication to fulfill any requirements by our clients to satisfactory. At Creative Biogene, industry-leading PV customization service is offered to all of our clients at competitive pricing with rapid turnaround time, ensuring an easy and trouble-free start of your research.
Recombinant viruses are especially useful for gene transfer and protein expression in vivo and in vitro. During viral vector production, particles may be generated which are replication competent through recombination with host sequences or by contamination. The probability of producing replication competent virus, although low, increases with each successive amplification. Therefore, development of a sensitive assay for detection of replication-competent recombinant viruses in large-scale vector preparations is needed.
Genomic engineering in cell lines is a versatile tool for studying gene function, designing diseases models, biopharmaceutical research, drug discovery and many other applications. CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats)/Cas9 systems is a newly developed yet the most popular method for genome editing. It has been widely used in current biology, functional genome screening, cell-based human hereditary disease modeling, epigenomic studies and visualization of cellular processes.
Creative Biogene has established various plant-based protein expression systems to provide recombinant protein production service for global clients. Using our state-of-art platform, we offer a complete service that begins from a specific gene to the purified protein, as well as different options for protein expression systems. We devote to accelerate clients' research in the development and manufacture of therapeutic drugs, vaccines, and diagnostics.
Creative Biogene is one of the leading biotechnology companies which can provide high quality subtractive cDNA library construction services for customers worldwide. Creative Biogene's proprietary, self-subtraction/ tissue-tissue subtraction techniques and highly experienced staffs are able to irrelevant abundant sequences 10-100 folds, making it easier to find rare or low-expressed target genes. Besides, with years of experience in the cDNA library construction field, Creative Biogene can provide the most affordable subtractive cDNA library construction services with fastest turnaround time to satisfy your downstream needs.
Creative Biogene is one of the leading biotechnology companies which has extensive expertise and experience in providing custom vector construction and modification service for customers worldwide. Creative Biogene's sophisticated equipments, advanced technologies and highly experienced staffs are available to provide you with the complete custom vector construction and modification service, including sequences analysis, gene synthesis & cloning, DNA fragment assembling, DNA sequencing, site directed mutagenesis, and plasmid production, etc. Using fast and efficient construction and modification methods, Creative Biogene can quickly create novel custom vector constructs for large or small projects.